Morphometric analysis of forewing venation does not consistently differentiate the leafhopper tribes Typhlocybini and Zyginellini

Tribes of the leafhopper subfamily Typhlocybinae have traditionally been defined based on differences in hind wing venation, but forewing venation also differs among some tribes. Here we used geometric morphometric analysis to determine whether previously recognized tribes can be distinguished configuration veins. Focusing apical area male right forewing, 76 semi-landmarks six curves corresponding individual veins were measured for representatives four and data analyzed using principal component (PCA), canonical variable (CVA), UPGMA clustering analysis. The study showed that mainly occur RP, MP′, MP″ + CuA′. PCA, CVA, cluster three distinct clusters representing Empoascini, Erythroneurini, Typhlocybini (sensu lato) failed distinguish stricto) from Zyginellini, which has considered as either separate tribe or a synonym by recent authors. results show Typhlocybinae, agree with molecular phylogenetic analyses, indicating Zyginellini is derived within Typhlocybini.